Sequencing Calculator v 3.0.0

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Edit Type SureMASTR kit Name amplicon pool No. of reads No. of samples
  • The TTC and VAFTumor values cannot be changed anymore as soon as the number of samples has been moved away from its default value of 0. The TTC and VAFTumor values cannot be changed anymore as soon as the number of samples has been moved away from its default value of 0.

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Technical Guide

Sequencing Calculator v 3.0.0 Report

Date:

MPS System:

Flowcell:

Remaining Capacity:

PhiX Control:

Mutation type Name amplicon pool No. of samples Min coverage per var. all. TTC (%) VAFTumor (%) VAFsample (%) Min covbase Mean covamp Min covamp No. of Reads Volume per amplicon pool (μl) Message ID
Message ID Message

Steps for preparation of amplicon pool per sequencing run:

  1. Prepare amplicon pools as indicated in the Instructions For Use.
  2. Bring all amplicon pools to the same molarity and vortex well.
  3. Refer to table for required volume per amplicon pool.
For additional information please contact: genomics_tech_europe@agilent.com [EU], ngs.support@agilent.com [US-Canada] or general [contact form]
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